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crop seq opti dsred vector backbone  (Addgene inc)


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    Addgene inc crop seq opti dsred vector backbone
    Crop Seq Opti Dsred Vector Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crop+seq+opti+dsred/CROP-seq-opti+(Plasmid+%23106280)/pm41611887-914-1-7
    Average 95 stars, based on 44 article reviews
    crop seq opti dsred vector backbone - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Transduction:

    Article Title: Developmental convergence and divergence in human stem cell models of autism.
    Article Snippet: Human embryonic kidney 293T (HEK293T) cells obtained from American Type Culture Collection (lines CRL-3216 and TIB-202) were expanded in DMEM, 10% FBS and 1% penicillin and streptomycin (HEK medium). .. Then 5 × 106 HEK293T were plated onto each of 2 poly-ornithine (5 μg ml−1) coated 10-cm plates for 24 h. Media from both plates was replaced with Opti-MEM before cells from 1 10-cm plate were transduced with a lentiviral vector (pLV-KRAB-dCas9 (Addgene no. 71236) or CROP-seq-opti-dsRed (Addgene no. 201999)) while the other plate of cells was transfected with 15 μg of the pooled plasmid library, 15 μg of psPAX2 and 1.5 μg of VSV-G with lipofectamine 3000 following the manufacturer’s instructions. ..

    Article Title: Developmental convergence and divergence in human stem cell models of autism
    Article Snippet: Human embryonic kidney 293T (HEK293T) cells obtained from American Type Culture Collection (lines CRL-3216 and TIB-202) were expanded in DMEM, 10% FBS and 1% penicillin and streptomycin (HEK medium). .. Then 5 × 10 6 HEK293T were plated onto each of 2 poly-ornithine (5 μg ml −1 ) coated 10-cm plates for 24 h. Media from both plates was replaced with Opti-MEM before cells from 1 10-cm plate were transduced with a lentiviral vector (pLV-KRAB-dCas9 (Addgene no. 71236) or CROP-seq-opti-dsRed (Addgene no. 201999)) while the other plate of cells was transfected with 15 μg of the pooled plasmid library, 15 μg of psPAX2 and 1.5 μg of VSV-G with lipofectamine 3000 following the manufacturer’s instructions. ..

    Transfection:

    Article Title: Developmental convergence and divergence in human stem cell models of autism.
    Article Snippet: Human embryonic kidney 293T (HEK293T) cells obtained from American Type Culture Collection (lines CRL-3216 and TIB-202) were expanded in DMEM, 10% FBS and 1% penicillin and streptomycin (HEK medium). .. Then 5 × 106 HEK293T were plated onto each of 2 poly-ornithine (5 μg ml−1) coated 10-cm plates for 24 h. Media from both plates was replaced with Opti-MEM before cells from 1 10-cm plate were transduced with a lentiviral vector (pLV-KRAB-dCas9 (Addgene no. 71236) or CROP-seq-opti-dsRed (Addgene no. 201999)) while the other plate of cells was transfected with 15 μg of the pooled plasmid library, 15 μg of psPAX2 and 1.5 μg of VSV-G with lipofectamine 3000 following the manufacturer’s instructions. ..

    Article Title: Developmental convergence and divergence in human stem cell models of autism
    Article Snippet: Human embryonic kidney 293T (HEK293T) cells obtained from American Type Culture Collection (lines CRL-3216 and TIB-202) were expanded in DMEM, 10% FBS and 1% penicillin and streptomycin (HEK medium). .. Then 5 × 10 6 HEK293T were plated onto each of 2 poly-ornithine (5 μg ml −1 ) coated 10-cm plates for 24 h. Media from both plates was replaced with Opti-MEM before cells from 1 10-cm plate were transduced with a lentiviral vector (pLV-KRAB-dCas9 (Addgene no. 71236) or CROP-seq-opti-dsRed (Addgene no. 201999)) while the other plate of cells was transfected with 15 μg of the pooled plasmid library, 15 μg of psPAX2 and 1.5 μg of VSV-G with lipofectamine 3000 following the manufacturer’s instructions. ..

    Plasmid Preparation:

    Article Title: Developmental convergence and divergence in human stem cell models of autism.
    Article Snippet: Human embryonic kidney 293T (HEK293T) cells obtained from American Type Culture Collection (lines CRL-3216 and TIB-202) were expanded in DMEM, 10% FBS and 1% penicillin and streptomycin (HEK medium). .. Then 5 × 106 HEK293T were plated onto each of 2 poly-ornithine (5 μg ml−1) coated 10-cm plates for 24 h. Media from both plates was replaced with Opti-MEM before cells from 1 10-cm plate were transduced with a lentiviral vector (pLV-KRAB-dCas9 (Addgene no. 71236) or CROP-seq-opti-dsRed (Addgene no. 201999)) while the other plate of cells was transfected with 15 μg of the pooled plasmid library, 15 μg of psPAX2 and 1.5 μg of VSV-G with lipofectamine 3000 following the manufacturer’s instructions. ..

    Article Title: Developmental convergence and divergence in human stem cell models of autism
    Article Snippet: Human embryonic kidney 293T (HEK293T) cells obtained from American Type Culture Collection (lines CRL-3216 and TIB-202) were expanded in DMEM, 10% FBS and 1% penicillin and streptomycin (HEK medium). .. Then 5 × 10 6 HEK293T were plated onto each of 2 poly-ornithine (5 μg ml −1 ) coated 10-cm plates for 24 h. Media from both plates was replaced with Opti-MEM before cells from 1 10-cm plate were transduced with a lentiviral vector (pLV-KRAB-dCas9 (Addgene no. 71236) or CROP-seq-opti-dsRed (Addgene no. 201999)) while the other plate of cells was transfected with 15 μg of the pooled plasmid library, 15 μg of psPAX2 and 1.5 μg of VSV-G with lipofectamine 3000 following the manufacturer’s instructions. ..



    Similar Products

    crop seq opti dsred vector backbone  (Addgene inc)
    95
    Addgene inc crop seq opti dsred vector backbone
    Crop Seq Opti Dsred Vector Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crop+seq+opti+dsred/CROP-seq-opti+(Plasmid+%23106280)/pm41611887-914-1-7
    Average 95 stars, based on 1 article reviews
    crop seq opti dsred vector backbone - by Bioz Stars, 2026-09
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    crop seq opti dsred  (Addgene inc)
    95
    Addgene inc crop seq opti dsred
    ( a ) Schema of CROP-Seq vector. ( b ) Example of gating for flow cytometry. Left shows a set of hNPCs not expressing virus used for gating compared with hNPCs expressing both dCas9-Krab <t>(GFP)</t> <t>and</t> <t>Crop-Seq-opti-dsRed</t> (TdTomato). Box in the upper right shows double positive cells selected for downstream analysis (~20% of live cell fraction). ( c ) QC measures for CRISPRi single cell libraries organized by gRNA negative (no gRNA detected in sequencing), Control gRNA positive, or Target gRNA positive. ( d ) Number of gRNA UMIs per cell. X-axis shows cut-off of 10 UMIs used to consider a cell gRNA positive (left). Number of of unique gRNAs expressed in each cell (x-axis). Only cells expressing gRNA for a single gene-target are retained for downstream analyses. ( e ) Single cell UMAPs of markers used to differentiate cycling and non-cyling NPCs ( f ) Single cell UMAP coloured by Target-gene presence ( g ) Proportion of cells uniquely expressing gRNAs barcodes for each target within each experiment (n = 18 per Gene comprised of 6 technical replicates, 3 gRNAs barcodes per target, for Controls n = 108: 18 gRNAs across 6 technical replicates). TP53 show increased proportion compared to non-targeting controls (NTCs). Boxplots a-c show: centre, median, lower hinge – 25% quantile, upper hinge – 75% quantile, lower whisker – smallest observation greater than or equal to lower hinge –1.5× interquartile range, upper whisker – largest observation less than or equal to upper hinge +1.5× interquartile range. ***Family wise corrected p < 0.005, Dunnets post-hoc contrast of linear model.
    Crop Seq Opti Dsred, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crop+seq+opti+dsred/CROP-seq-opti+(Plasmid+%23106280)/pmc12999519-407-49-50
    Average 95 stars, based on 1 article reviews
    crop seq opti dsred - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) Schema of CROP-Seq vector. ( b ) Example of gating for flow cytometry. Left shows a set of hNPCs not expressing virus used for gating compared with hNPCs expressing both dCas9-Krab (GFP) and Crop-Seq-opti-dsRed (TdTomato). Box in the upper right shows double positive cells selected for downstream analysis (~20% of live cell fraction). ( c ) QC measures for CRISPRi single cell libraries organized by gRNA negative (no gRNA detected in sequencing), Control gRNA positive, or Target gRNA positive. ( d ) Number of gRNA UMIs per cell. X-axis shows cut-off of 10 UMIs used to consider a cell gRNA positive (left). Number of of unique gRNAs expressed in each cell (x-axis). Only cells expressing gRNA for a single gene-target are retained for downstream analyses. ( e ) Single cell UMAPs of markers used to differentiate cycling and non-cyling NPCs ( f ) Single cell UMAP coloured by Target-gene presence ( g ) Proportion of cells uniquely expressing gRNAs barcodes for each target within each experiment (n = 18 per Gene comprised of 6 technical replicates, 3 gRNAs barcodes per target, for Controls n = 108: 18 gRNAs across 6 technical replicates). TP53 show increased proportion compared to non-targeting controls (NTCs). Boxplots a-c show: centre, median, lower hinge – 25% quantile, upper hinge – 75% quantile, lower whisker – smallest observation greater than or equal to lower hinge –1.5× interquartile range, upper whisker – largest observation less than or equal to upper hinge +1.5× interquartile range. ***Family wise corrected p < 0.005, Dunnets post-hoc contrast of linear model.

    Journal: Nature

    Article Title: Developmental convergence and divergence in human stem cell models of autism

    doi: 10.1038/s41586-025-10047-5

    Figure Lengend Snippet: ( a ) Schema of CROP-Seq vector. ( b ) Example of gating for flow cytometry. Left shows a set of hNPCs not expressing virus used for gating compared with hNPCs expressing both dCas9-Krab (GFP) and Crop-Seq-opti-dsRed (TdTomato). Box in the upper right shows double positive cells selected for downstream analysis (~20% of live cell fraction). ( c ) QC measures for CRISPRi single cell libraries organized by gRNA negative (no gRNA detected in sequencing), Control gRNA positive, or Target gRNA positive. ( d ) Number of gRNA UMIs per cell. X-axis shows cut-off of 10 UMIs used to consider a cell gRNA positive (left). Number of of unique gRNAs expressed in each cell (x-axis). Only cells expressing gRNA for a single gene-target are retained for downstream analyses. ( e ) Single cell UMAPs of markers used to differentiate cycling and non-cyling NPCs ( f ) Single cell UMAP coloured by Target-gene presence ( g ) Proportion of cells uniquely expressing gRNAs barcodes for each target within each experiment (n = 18 per Gene comprised of 6 technical replicates, 3 gRNAs barcodes per target, for Controls n = 108: 18 gRNAs across 6 technical replicates). TP53 show increased proportion compared to non-targeting controls (NTCs). Boxplots a-c show: centre, median, lower hinge – 25% quantile, upper hinge – 75% quantile, lower whisker – smallest observation greater than or equal to lower hinge –1.5× interquartile range, upper whisker – largest observation less than or equal to upper hinge +1.5× interquartile range. ***Family wise corrected p < 0.005, Dunnets post-hoc contrast of linear model.

    Article Snippet: Then 5 × 10 6 HEK293T were plated onto each of 2 poly-ornithine (5 μg ml −1 ) coated 10-cm plates for 24 h. Media from both plates was replaced with Opti-MEM before cells from 1 10-cm plate were transduced with a lentiviral vector (pLV-KRAB-dCas9 (Addgene no. 71236) or CROP-seq-opti-dsRed (Addgene no. 201999)) while the other plate of cells was transfected with 15 μg of the pooled plasmid library, 15 μg of psPAX2 and 1.5 μg of VSV-G with lipofectamine 3000 following the manufacturer’s instructions.

    Techniques: Plasmid Preparation, Flow Cytometry, Expressing, Virus, Single Cell, Sequencing, Control, Whisker Assay